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Intestine samples were incubated overnight with Histodenz (Sigma, #D2158) at room temperature (RT)

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sEH protein was also detected in the liver by immunofluorescence, which co-localized with the hepatocyte marker albumin and was increased by AngII infusion (Fig

These results suggest that epigenetic inactivation of GSTP1 plays an important role in the development of HCC and exposure to environmental carcinogens may be related to altered methylation of genes involved in hepatocarcinogenesis (Zhang et al., 2012)