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PCR reactions were carried out using 0.5 L of gene-specific cDNA samples and that of 16S rRNA as templates with 1 mM final concentration of each of the forward and reverse RT primers (Additional file 12: Table S5) and 4 L of 2 SYBR green PCR mix in a final volume of 10 L

Adenosylcobalamin (also called cobamamide) is the other active form of B12, found primarily within mitochondria where it supports energy metabolism

In the Ras pathway, Erk1/2 as two mitogen-activated protein kinases to transmit mitotic signals, and the HGF/c-Met signaling pathway phosphorylates and activates Erk1/2 after PHx (Spector et al., 1997

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Mitochondria are the cell's energy organelles

This is consistent with tolerance of K1A substitution in human Amy (hAmy) for cAMP potency at AMYRs 18