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About 100 mg (dry weight) of immobilization carriers were collected and weighed in a 15 mL tube, N,N -dimethyl-4-aminopyridine (DMAP) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) and N,N' -disuccinimidyl carbonate (DSC) (Tokyo Chemical Industry, Tokyo, Japan) were weighed to 1.51- and 3.16-times the weight of the immobilization carrier, respectively