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J Biol Chem 254(16):75587560 Gu J, Sun X, Wang G, Li M, Chi M (2011) Icariside II enhances Nrf2 nuclear translocation to upregulate phase II detoxifying enzyme expression coupled with the ERK, Akt and JNK signaling pathways

Incremental Addition of NaCl: 2000 L of residual NaCl concentrations were added incrementally under the same incubation conditions: 500 L of 0.5 M NaCl (added in 250 L portions every 30 min)

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These peaks were then clustered based on retention time and chromatographic profile to identify groups of unique chemical species and their parent peak (because each compound can give many LCMS features that are isotopic peaks or adducts of the parent compound)