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Neuropathic Pain: Delving into the Oxidative Origin and the Possible Implication of Transient Receptor Potential Channels

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10.1007/s11064-020-03173-1 Neurochem Res

Immunofluorescence in primary cortical neurons Micro Coverglass #1.5 coverslips (Electron Microscopy Sciences) coverslips were washed in 2 M HCl overnight at 55 C, washed 5 times in sterile H 2 O, then pre-coated with poly-D-lysine (50 g/mL) overnight at 37 C, then washed with distilled water three times and air-dried at room temperature for at least 2 h