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For immunohistochemistry (IHC) labeling, heat-mediated epitope retrieval was performed in 0.01 m sodium citrate buffer, pH 6, containing 0.05% Tween-20, which was heated in a microwave oven to 95C for 30 min

Slides were deparaffinized in xylene, rehydrated through a graded ethanol series, and stained with Nissl staining solution (Beyotime, C0117, China) for 5 min

In some cases, additional tests such as blood tests or biopsies may be required to rule out other conditions

Ultimately, 41 articles were included in the analysis (Fig

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Subsequent analysis indicated elevated expression of COX2 and -SMA in the fibrotic group, as observed through immunofluorescence staining (Fig