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In a state of mitochondrial dysfunction, partially metabolized fatty acids can build up inside the mitochondria, forming toxic acyl-CoA compounds that inhibit essential enzymes and cause severe oxidative stress

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After 0.5 h, the radio activity initially held by [1- 14 C] palmitate was recovered on labelled short molecules released from the -oxidative cycle and soluble in perchloric acid (acid-soluble products, ASP)

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