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doi: 10.1038/nature13148 15 DowdleWENyfelerBNagelJEllingRALiuSTriantafellowEet al

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H 2 S has shown therapeutic potential for ALS by inhibiting SOD1 aggregation and countering oxidative modifications
The column-bound fusion proteins were eluted by adding 2025 mM reduced glutathione in binding buffer and adjusting the pH to pH 8.0