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All PCR products were cut with KpnI/NotI and then used to exchange the corresponding GFP sequence from pEGFP-N1

For extended experiments, opened vials may be refrigerated at 28C and typically discarded within 28 days according to laboratory standards

For a broader look at what B12 does beyond energy including its role in nerves, methylation, and brain health see our complete vitamin B12 benefits guide

hemorrhagic stroke and cognitive effects)

Detection wavelengths were set at 220 nm for ITC-Cys and ITC-Cyclic-Cys conjugates, 280 nm for ITC-CysGly conjugates, and 240 nm for ITC-Lys conjugate
