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In addition, the expressions of Bax (Figure 4B), Bcl-2 (Figure 4C), and Casp3 (Figure 4D) genes, which are involved in the cell death signaling pathway (apoptosis), were measured, and the results indicated overexpressions of Bax and Casp3 genes and a downregulation of Bcl-2 in the 6-OHDA-exposed cells

The BBB is an essential interface between CNS and periphery and is composed of endothelial cells, which are tightly connected to each other by TJs and adherents junctions (AJs) [119, 141,142,143]

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For instance, MST was found to increase gray matter volume (GMV) in the bilateral paracentral lobule 9 , but ECT studies found restricted volume changes in the hippocampusamygdala complex 10,11 or widespread alterations in the brain 12

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In-silico docking and molecular dynamics corroborate the complex preferentially binding within the minor groove across a six-base pair binding site with binding predominantly stabilised by electrostatic forces augmented by van der Waals interactions that are otherwise lacking when the complex resides in the major groove