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Total protein (25 g/lane) was separated using 12% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) at 80110 V for 150 min and then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, Billerica, MA, USA) at 100 V for 2 h

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Protein samples (1525 g) were separated on 812% pre-made sodium dodecyl sulfate-polyacrylamide gels (Thermo Scientific) and transferred onto polyvinylidene difluoride (Merck Millipore) membranes using absolute methanol (Thermo Fisher Scientific)