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One relevant observation, he said, is that ketamine exerts its antidepressant effects across a very narrow range of doses

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Samples were run on Tris-tricine or Tris-HCl SDS-PAGE gels and transferred to 0.45 m Immobilon-P Polyvinylidene Fluoride (PVDF) (Millipore) for Western blotting using ECL Prime substrate (GE Healthcare)

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Crucially, the functional significance of this lactate shuttle has been strongly supported by interventional studies