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Bacterial concentration was estimated based on a pre-established standard curve correlating OD with counts (CFU/mL)

however circulating levels also are dependent on the proteins that bind IGF-1 in the circulation

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To distinguish the fraction of BCA signal in AMW extracts arising from bona fide proteins versus non-proteinaceous factors, we filtered crude extracts through a 3 kDa filter

doi: 10.4161/cc.8.23.10238 29 FuYLiuSYinSNiuWXiongWTanMet al

This paper reviews recent data on the mechanisms and pathways that integrate gut-brain-kidney signaling and communication, advances in our understanding of this axis, and potential diagnostic and prognostic biomarkers and interventions for CKD