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Each sample was fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membrane

37 A typical workflow for synthetic biology-driven natural product discovery is shown in Figure 6

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This residue was studied using site-directed mutagenesis, and when Tyr was replaced by different amino acids, GST has lost at least 90% its specific activity [2224]

The calibration plot for glutathione detection is calculated by absorbance difference at 652 nm, which ranged from 0.5 to 10 M with a detection limit of 0.2 M