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Dilutions in culture media supplemented with 10% fetal calf serum were made immediately before the experiments at the indicated concentrations

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doi:10.1002/jcp.1041040211 23

Living cells workstation The cells were seeded into a 35 mm confocal dish for 24 h culture and OA treatment, and then were washed 3 times with PBS and then added to the DMEM culture containing BODIPY (1:1000, v/v), followed by incubation for 20 min at 37 C

While the redox and electrophilic response cis elements have been identified, less has been done to identify the signaling mechanisms that activate the transcription factors that bind to those elements

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