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In the brain, extracellular Glu content is maintained at extremely low levels because Glu may exert excitotoxicity in some neurons expressing NMDA-type glutamate receptors [163]

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Cells were cultured in DMEM/F12 medium containing 4 mM L-glutamine ( 13 C 5 , 99%), 10% FBS, and 1% penicillin-streptomycin for 24 h before cell pellet collection