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Positive controls should be a known protein that has already undergone purification and analysis, and that has a high-confidence set of interacting proteins identified by counterpart binding assays
The ratio of ruthenium released from the nanoparticles was calculated as the percentage of cumulative ruthenium in the dialysate relative to the total amount of ruthenium present in the nanoparticles

PLoS One 10(3):e0120879

F.ZhaoY.DucklesS

Figure 6 Protein Expressions of Apoptotic and Inflammatory Markers Western blotting revealed that the protein expressions of the cleaved forms of PARP and caspase 3 were significantly upregulated in the HFC group, and these changes were not eliminated in the KD group

Sweiss, University of Illinois at Chicago, United States Updates Copyright 2020 Gerke
