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Change of redox status and modulation by thiol replenishment in retinal photooxidative damage

22 Therefore, to determine whether the metabolite induces proteotoxicity in part through UPS inhibition, we reduced proteasome function with a range of MG132 concentrations and identified that (at concentrations above 5 M) MG132 induces neurodegeneration in the presence of -synuclein and that this neurotoxicity epistatically regulates metabolite-induced neurotoxicity (Supplementary Figure S4a)

Fluorescence microscopy Worms were immobilized with 3 mM levamisole and mounted on 2% agarose pads on a microscopic slide