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Description
The peptide consists of three amino acids linked in a specific sequence that demonstrates high affinity for copper binding

coli , gene synthesized (Biomatik), and inserted into pET28a vector using Eco R1 and Nde 1 sites in frame with an N-terminal 6x-His tag using a previously described cloning method (Schenck et al., For site directed mutagenesis, plasmid template was diluted 100-fold, mixed with 0.04 U/L Phusion DNA polymerase (Thermo), 0.2 mM dNTP's, 0.5 M forward and reverse mutagenesis primers and 1x Phusion reaction buffer (Thermo), and then placed in a thermocycler for 98C for 30 s followed by 20 cycles of 10 s at 98C, 20 s at 70C, 4.5 min at 72C with a final extension at 72C for 10 min

Lutgendorff F, Nijmeijer RM, Sandstrom PA, Trulsson LM, Magnusson KE, Timmerman HM

The coverage is automatic, which means there is no need to enrol or register for OHIP+

GHK-Cu skincare products are formulas that use copper peptide, also known as copper tripeptide-1
Sep-Oct 2012;29(5):599-615