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In vitro studies, over-expression of the IGF1R has been identified as a typical hallmark of many types of tumors (Werner et al., 2012)

Czeisler CA, Shanahan TL, Klerman EB, Martens H, Brotman DJ, Emens JS, et al

The cells were diluted to a certain density and then moved to polylysine (Sigma, P1399)-coated plates for culturing at 37 C in a 5% CO 2 incubator

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If supplementation is advised, oral cyanocobalamin at doses of 50150 micrograms daily is typically used for diet-related deficiency prevention, while intramuscular hydroxocobalamin is the standard treatment in the UK for pernicious anaemia or malabsorption conditions