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5-Amino-1MQ is a small-molecule quinolinium compound that has been the focus of metabolic and enzymatic research, particularly in relation to its potential role in inhibiting nicotinamide N-methyltransferase (NNMT), an enzyme implicated in metabolic regulation and obesity-related studies

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Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

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