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Plates were stirred and laccase activity was assayed at room temperature in a plate reader SpectraMax ABS Plus (Molecular Devices)

For example, a single 150 g serving of mackerel is estimated to provide over 2000 mg of EPA and DHA, which would meet the entire weekly requirement for these n -3 fatty acids (Reference Rincn-Cervera, Gonzlez-Barriga and Romero107)