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1 g (for zebrafish larvae and mouse tissue samples) or 500 ng (for adult zebrafish tissue samples) of total RNA were treated with RQ1 RNase-Free DNase (M6101, Promega) and used for cDNA synthesis, with the High-Capacity cDNA Reverse Transcription Kit (4368814, ThermoFisher)

Glutathione also plays a role in processing medications and cancer-causing compounds (carcinogens), and building DNA, proteins, and other important cellular components

Novel Aspects of Glutamine Synthetase in Ammonia Homeostasis

doi: 10.1016/j.nbd.2015.05.009 356 Halon-GolabekMBorkowskaAHerman-AntosiewiczAAntosiewiczJ

Post hoc analysis of LEADER data revealed a persistent increase in the overall risk of biliary tract-related events and gallbladder diseases, consistently observed across four categories: uncomplicated gallbladder stones, complicated gallbladder stones, cholecystitis with/without gallbladder stones, and biliary obstruction [34]
