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Description
That lets researchers use it several times without the risk of unclean samples and keep experiments clean

Regular monitoring of platelets and blood counts required

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Chapman LE, Darling AL, Brown JE (2015) The association between the biguanide drug metformin and vitamin B 12 deficiency in diabetic patients: a systematic review

Neurocrit Care
Increased expression of the immediate response gene, early growth response gene-1 (egr-1), was shown in Caco-2 cells in vitro (Tkalcevic et al., 2007)
