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At 24 h post-transfection, the total RNA was isolated from LMH cells, and the relative mRNA abundance was quantified to assess transfection efficiency [27]

Pharmacogn Mag 13:S658S662

This same enzyme was subsequently identified by two other laboratories from database searches of proteins containing the conserved G X S X G pentapeptide motif and alpha/beta-hydrolase fold, and was named adipose triglyceride lipase (ATGL) (145) or iPLA2 (53, 145)

Limitations of the study One of the key limitations of this study is that the findings are based on preliminary data from publicly available sources

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