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Detailed methods for DNA extraction, PCR amplification, and Sanger sequencing are outlined previously 45

The expiration date on an unopened vial ensures its sterility and the stability of the solution

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The activation of calpain-2 is associated with numerous downstream consequences, including the cleavage of cytoskeletal proteins into stable proteolytic fragments that can be captured as fluid-based biomarkers (Saatman et al., 2010)

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