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Perls staining showed no significant change in Fe but markedly increased Fe deposition

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Preliminary experiments The purposes of the experiments were to determine the optimum pH of the buffer solution for non enzymatic and enzymatic reactions of monobromobimane (MBB) with glutathione (GSH) as well as the quenching effect of the presence of excess MBB in a typical reaction mixture on the fluorescence of the reaction product

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this observation can be a clue to use cysteine levels to predict the therapy response and the behaviour of tumours