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Alternative treatments should be considered by pregnant women, nursing mothers, and people with asthma, sulfite allergies, or specific kidney/liver conditions

Redox assays and immunoblotting At the time of killing, the livers were perfused with saline (cardiac portal), excised, cut into ~0.3 g pieces and pieces were either fixed in neutral-buffered formalin for paraffin-embedding and histology or snap-frozen in liquid nitrogen and stored at 80 C 23

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