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NAD+/NADH ratio assays, SIRT1 activity assays, and mitochondrial respiration measurements (such as oxygen consumption rate via extracellular flux analysis) are standard methods for studying this combination's proposed effects in cell-based models, typically comparing single-compound and combined-compound treatment conditions against untreated controls

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Animal studies suggest that reducing NNMT activity may: increase cellular energy expenditure improve metabolic efficiency reduce fat accumulation influence NAD metabolism These findings have made NNMT a potential therapeutic target for metabolic disease research