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Comparison of apo-PBP1b and moenomycin-bound PBP1b structures shows that binding of this lipid II mimetic at the GT site is accompanied by a rigid-body tilting and rotation of UB2H and TP domains together towards the GT domain, suggesting that reorientation of GT and UB2H domains can be used as proxy for GT-TP motion 40,41,42 (SI Fig