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This molecule was duplexed and amplified by 5 cycles of PCR using Phusion HF polymerase (New England Biolabs, NEB), then inserted into blunt II TOPO vector (Invitrogen) to generate a plasmid for electroporation efficiency control (pSSBR)

Peroxynitrite The peroxynitrite anion is formed through the reaction between two radicals, O 2 and NO (Equation (1)), which occurs at rates that are controlled by diffusion [30]

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