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[11] In other organisms [edit] The LPL gene is highly conserved across vertebrates

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Synchronized L4 animals were collected, rinsed 3 times with ice-cold ddH2O, and immersed in RFB solution (160 mM KCl, 40 mM NaCl, 20 mM EGTA, 10 mM Spermidine-HCl, 30 mM Na-PIPES, pH 7.4, and 50% methanol) supplemented with 1% PFA (Cat# 15710

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For the pharmacological intervention in the chronic HUA experiment, 75 mice were randomly divided into five groups: the blank group, HUA model group, LC group, TMZ group and allopurinol (AP, Sigma, A8003) group