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Shots may be weekly, bi-weekly, or monthly depending upon desired results.

3.8 GHK-Cu inhibited the expression of RORt in DSS-induced colitis Western blot analysis showed that treatment with GHK-Cu significantly reduced DSS-induced RORt expression (Figure 9), implying Th17 cell inhibition contributes to its therapeutic effect

This means that high serum B12 levels can persist for some time after large supplemental doses

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If negative vitamin B12 supplement side effects persist, consider decreasing your dosage, or consult with your doctor