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S2 was ultracentrifuged at 165,000 g for 2 h at 4 C, generating supernatant S3 (cytosolic proteins) and pellet P3 enriched in light intracellular membranes (LM)

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[Google Scholar] [CrossRef] Hepburn DD, Xiao J, Bindom S, Vincent JB, ODonnell J (2003)

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She had no fever, weight loss, irritability, recent travel or known infectious contacts
