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Tumor spheroid viability assay K1 cells were incubated to form spheroids (~ 7 days) in ultra-low binding 96-well plates for viability assay and in ultra-low binding 24-well plate for imaging with PI staining

Glutathione synthesis The first step in de novo GSH synthesis involves the combination of cysteine with glutamate to produce -glutamylcysteine

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Proc Natl Acad Sci USA 94:41554160 Picaud S, Larsson HP, Wellis DP, Lecar H, Werblin F (1995) Cone photoreceptors respond to their own glutamate release in the tiger salamander