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doi: 10.1038/s41593-020-0624-8 122 HanX.LeiQ.LiuH.ZhangT.GouX

doi: 10.1016/j.jmb.2005.11.002 4 QuantiusJSchmoldtCVazquez-ArmendarizAIBeckerCElAgha EWilhelmJet al

These cell lines were maintained in Dulbeccos Modified Eagles Medium (DMEM) (Thermo Scientific, #12100061)low glucose for HepG2 (Thermo Scientific, #31600091), supplemented with 1 % Penicillin/Streptomycin (P/S) and 10 % Fetal bovine serum (FBS) (Natocor)

Co-treatment with Hyperbaric Oxygen and 0.3mM AA significantly increased cytotoxicity compared to all other treatments presumably by providing additional substrate for Hydrogen peroxide production

Of course, good sleep and daily stress management are also essential

These patients had all failed at least 1 year of standard treatment