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The GSH response with repeated exposures is also very interesting since the level of GSH in the ELF is established at a lower, but still protective level within the first 3 days of CS exposure

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The LC separation consisted of solvent A: H 2 O/0.1% formic acid and solvent B: methanol /0.1% formic acid with the following gradient: 10 min 3% acetonitrile, 10 min 30%, 20 min 70%, 20 min 100% with a flow rate of 4 l min 1 for 60 min