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Description
Approximately 0.3% of total T3 (TT3) and 0.03% of total T4 (TT4) circulate in the free or unbound state (i.e., free T3fT3 and free T4fT4) due to the noncovalent and rapidly reversible binding to plasma proteins [31]

Aust J Basic Appl Sci
Rahnasto-Rilla, M
BMC Clin Pathol 15:3 Watowich SS (2011) The erythropoietin receptor: molecular structure and hematopoietic signaling pathways

Metastasis 32 , 605622 (2015)

Samples were centrifuged at 6000 rpm for 15 min at room temperature, plasma was divided into four Eppendorf tubes and kept at -80 until analysis
