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For each assay, established enzyme specific inhibitors were included as positive controls for enzyme function and assay reproducibility

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The theoretical indirect loading efficiency of sulforaphane into exosomes was determined using the UV-vis spectrometry method (Jasco V-730 UV-visible spectrophotometer, Japan)

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Both excessive ROS accumulation and the disruption of cellular ROS neutralization are shown to play crucial roles in the development of various diseases and pathological processes