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3 Results 3.1 Establishment and validation of a zebrafish model of T2DM This study aimed to establish a metabolically stable zebrafish model of T2DM for in vivo drug screening

2010 Oct 26;107(43):18611-5

The design principle: Standard (often fails): His6 - TEV site - Target protein Better: His6 - TEV site - (GGS)2 - Target protein Even better: His6 - (GGS)2 - TEV site - (GGS)2 - Target protein Failure 2: Non-Specific Cleavage (The Protease Damages Your Protein) The problem: Proteases aren't perfectly specific

So those are my week two updates

Nicht fr den menschlichen Gebrauch, medizinische, tierrztliche oder diagnostische Anwendungen

Ikatan peptida adalah ikatan kimia penting yang memberikan stabilitas pada protein dan peptida