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Love DT, Barrett TJ, White MY, Cordwell SJ, Davies MJ, Hawkins CL (2016) Cellular targets of the myeloperoxidase-derived oxidant hypothiocyanous acid (HOSCN) and its role in the inhibition of glycolysis in macrophages

We then used co-IP assay to validate their association

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The efficiency of each primer was calculated using qPCR assays of cDNA dilution series and plotting the log10 of the concentration versus the crossing point threshold (C(t)) values and using the primer efficiency equation, (10(1/Slope) 1) 100) (Supplemental Table S11)