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For activity measurements, neurons (6-8 weeks in culture) were stimulated with 200 action potentials, 40 Hz 5 s using a field stimulation chamber RC-47FSLP (Warner Instruments) and imaged at physiological temperature (37 C) in an imaging buffer 88 containing 1.3 mM Ca 2+ with an epifluorescence microscope Nikon Eclipse Ti equipped with a 40X oil objective