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High dose vitamin E attenuates diabetic nephropathy via alleviation of autophagic stress

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Then, 5 l of proteinase K (20 mg ml 1 ) and 200 l of 2 elution buffer (20 mM Tris-HCl pH 8.0, 300 mM NaCl, 20 mM EDTA, 2% SDS and 10 mM DTT) was added to each sample for reverse crosslinking at 65 C overnight

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Do I need blood tests to monitor my B12 levels after starting injections