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Description

For digestion and linker cleavage, gelled samples were gently transferred into 6 well glass bottom plates (Cellvis) and treated with 10 mM DTT and Proteinase K (New England Biolabs) at 8 units/mL in digestion buffer (50 mM Tris (pH 8), 1 mM EDTA, 0.5% Triton X-100, 1 M NaCl, 0.8 M guanidine HCl) overnight at RT

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The elevated acetyl-CoA/propionyl-CoA ratio resulting from both even-chain and odd-chain fatty acid oxidation may have beneficial effects in alleviating metabolic alterations in PA