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When the cellular GSH levels were artificially lowered or raised, TRaQ-G probes reliably detected changes in GSH concentration

doi: 10.1042/BJ20061044

second, under conditions of significant depletion of endogenous cysteine (Cys) and GSH, NAC can also act as a direct antioxidant against certain oxidants (nitrogen dioxide and hypochlorous acid)

As too much oxidation and reduction of cellular compounds are highly disadvantageous, maintaining redox homeostasis is essential for plants' survival and growth [147]

Moreover, other routes of administration, such as intravenous, intranasal, and sublingual administration, can increase GSH levels [99]