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Pathophysiologic Mechanisms of Tobacco Smoke Producing Atherosclerosis

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Acknowledgements This project is supported by the National Natural Science Foundation of China (NSFC), 52273197 (B.Z.T.) and 52333007 (Z.Z.), National Key Research and Development Program of China, 2023YFB3810001 (Z.Z.), China Postdoctoral Science Foundation, 2023M733423 (W.-J.W.), Shenzhen Key Laboratory of Functional Aggregate Materials, ZDSYS20211021111400001 (B.Z.T.), the Science, Technology and Innovation Commission of Shenzhen Municipality, KQTD20210811090142053 (B.Z.T.), JCYJ20220818103007014 (B.Z.T.), GJHZ20210705141810031 (Z.Z.) and JCYJ2021324134613038 (Z.Z.), Shenzhen Medical Research Fund, A2403004 (W.-J.W.), and Longgang District Medical Key Discipline Construction Found (R.H.)

Through the primers we introduced KpnI and NotI restriction sites, which were used to ligate the PCR-amplified gene region into the multiple cloning site of the RNAi vector L4440

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