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Use a sterile, new needle with each injection

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Immunofluorescence staining was performed using primary antibodies against Perilipin-2 (PLIN2, Thermo Fisher Scientific) or HSL (Cell Signaling Technology, Danvers, MA), followed by Alexa Fluor 594- or 488-conjugated anti-rabbit IgG antibodies

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The primary preclinical evidence comes from mouse studies showing reduced body weight and adipose mass in diet-induced obese mice (2018), improved grip strength in aged mice (2024), and beneficial microbiome changes when combined with caloric restriction (2022)

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and CimaVax EGF, which induces antibodies against EGF to block the binding between EGF and its receptor EGFR (Brahmer 2013

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