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However, it remains to be investigated whether these agents or RNA molecules can effectively cross the blood-brain barrier and safely inhibit HIV-1 expression in microglia

doi: 10.1126/science.abq5622 [DOI] [PMC free article] [PubMed] [Google Scholar] 69.Bhagat D S, Pansare D N, Thorat B R, Gaikwad Digambar D., Chawla Pooja A., Bumbrah Gurvinder Singh

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Brondani M, Roginski AC, Ribeiro RT, de Medeiros MP, Hoffmann CIH, Wajner M, et al

The cells were then incubated with primary antibodies directed against -H2AX (Sigma, 05-636, dilution 1:100), CD31 (Abcam, ab215912, dilution 1:50), or -SMA (Abcam, ab5694, dilution 1:1000) overnight at 4 C on a shaker

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