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The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)
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Subsequently, the SePro was dissolved in water to obtain a SePro solution with a mass fraction of 8%, using alkaline protease, neutral protease, and papain
Salem et al., 2015) (Figure 4)
As our cells get flooded with the compounds vital for peak function, we experience boosts in areas like: Energy and mitigating fatigue Strengthening immune function Facilitating muscle repair Enhancing brain activity These wellness benefits translate to feeling sharper and healthier from cellular nourishment
Pande K, Chen C, Noble SM
