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The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 30 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 2: 20 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles Library 3: 10 M 8-oxo-dGTP, 3 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library

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Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells

doi: 10.1016/j.jchromb.2020.122188

Half of the trials were congruent (24/48), where the numbers shown matched the number of ciphers (e.g., 333) and half of them were incongruent (e.g., 222)

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